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KMID : 0617319970060010061
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Journal of Pharmacetical Sceiences Ewha Womans University
1997 Volume.6 No. 1 p.61 ~ p.66
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Improvement of Carbapenem Antibiotics Productivity in S. cattleya by Transformation
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Abstract
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Streptomyces cattleya is a producer of carbapenem antibiotics, thienamycin and N-a-cetylthienamycin, which have potent and broad-spectrum antibacterial activities. We studied on strain improvement for antibiotic productivity of S. cattleya by transformation technique which employed S.cattleya protoplasts and chromosomal DNAs of glutamic acid producers: Corynebacterium glutamicum and Arthrobacter simplex. 150 Transformant strains were cultured and bioassayed using Bacillus subtilis and Staphylococcus aureus as test organisms. 8.7% of transformants tested showed 1.4~2.6 fold higher productivities than wild type which produced 1.61±0.67 ㎍/㎖. The best transformant produced 8.36±2.84 ㎍/㎖ carbapenems.
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